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Cleaved Caspase 3; p17 Monoclonal antibody

Cleaved Caspase 3; p17 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2F7B8

Cat no : 68773-1-Ig

Print datasheet

Synonyms

CASP3, Caspase3, Caspase 3, CASP-3, CASP 3

验证数据展示

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of BV-2 using 68773-1-Ig

    IF Staining of BV-2 using 68773-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed 50 μM Cisplatin (18 hours) treated BV-2 cells using Cleaved Caspase 3; p17 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • FC experiment of Jurkat using 68773-1-Ig

    FC experiment of Jurkat using 68773-1-Ig

    1x10^6 non-treated Jurkat cells (dash lines) and staurosporine treated Jurkat cells (solid lines) were intracellularly stained with 0.1 μg Cleaved Caspase 3; p17 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA .

  • FC experiment of Jurkat using 68773-1-Ig

    FC experiment of Jurkat using 68773-1-Ig

    1x10^6 non-treated Jurkat cells (dash lines) and staurosporine treated Jurkat cells (solid lines) were intracellularly stained with 0.2 μg Cleaved Caspase 3; p17 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA .

  • FC experiment of BV-2 using 68773-1-Ig

    FC experiment of BV-2 using 68773-1-Ig

    1x10^6 non-treated BV-2 cells (dash lines) and cisplatin treated BV-2 cells (solid lines) were intracellularly stained with 0.2 μg Cleaved Caspase 3; p17 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA .

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 68773-1-Ig

    IP experiment of Jurkat using 68773-1-Ig

    IP result of anti-Cleaved Caspase 3; p17 (IP:68773-1-Ig, 4ug; Detection:68773-1-Ig 1:5000) with Staurosporine treated Jurkat cells lysate 720 ug.

  • IHC staining of human lymphoma using 68773-1-Ig

    IHC staining of human lymphoma using 68773-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 68773-1-Ig (Cleaved Caspase 3; P17 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 68773-1-Ig

    IHC staining of human lymphoma using 68773-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 68773-1-Ig (Cleaved Caspase 3; P17 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 68773-1-Ig

    IHC staining of human ovary cancer using 68773-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68773-1-Ig (Cleaved Caspase 3; P17 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of Jurkat using 68773-1-Ig

    IHC staining of Jurkat using 68773-1-Ig

    Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Jurkat using 68773-1-Ig

    IF Staining of Jurkat using 68773-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed non-treated and 1 μM Staurosporine (1/3/6 hours) treated Jurkat cells using Cleaved Caspase 3; p17 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO. RGAM002 ).

  • IF Staining of HSC-T6 using 68773-1-Ig

    IF Staining of HSC-T6 using 68773-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed non-treated and 1 μM Staurosporine (1/3/6 hours) treated HSC-T6 cells using Cleaved Caspase 3; p17 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004).

经过测试的应用

Positive WB detected inJurkat cells, A2780 cells, BV-2 cells, HepG2 cells, HSC-T6 cells, Ramos cells
Positive IP detected inStaurosporine treated Jurkat cells
Positive IHC detected inhuman lymphoma tissue, Jurkat cells
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in1 μM Staurosporine (1/3/6 hour) treated Jurkat cells, 50 μM Cisplatin (18 hours) treated BV-2 cells, 1 μM Staurosporine (1/3/6 hour) treated HSC-T6 cells
Positive FC (Intra) detected in1 μM Staurosporine (3 hours) treated Jurkat cells, 50 μM Cisplatin（18h）treated BV-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68773-1-Ig targets Cleaved Caspase 3; p17 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name caspase 3, apoptosis-related cysteine peptidase
Synonyms CASP3, Caspase3, Caspase 3, CASP-3, CASP 3
Calculated Molecular Weight 32 kDa
Observed Molecular Weight17 kDa
GenBank Accession NumberNM_032991
Gene Symbol Caspase 3
Gene ID (NCBI) 836
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42574
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17 of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3, it can recognize the 17 kDa cleaved-caspase 3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).

实验方案

Product Specific Protocols
WB protocol for Cleaved Caspase 3; p17 antibody 68773-1-IgDownload protocol
IHC protocol for Cleaved Caspase 3; p17 antibody 68773-1-IgDownload protocol
IF protocol for Cleaved Caspase 3; p17 antibody 68773-1-IgDownload protocol
IP protocol for Cleaved Caspase 3; p17 antibody 68773-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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