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Cleaved Caspase 3; p17 Monoclonal antibody

Cleaved Caspase 3; p17 Monoclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2F7B8

Cat No : 68773-1-Ig

Print datasheet

Synonyms

Apopain, CASP 3, CASP3, Caspase 3, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

验证数据展示

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68773-1-Ig

    WB analysis using 68773-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3; p17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 68773-1-Ig

    IF Staining of Jurkat using 68773-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed 1 μM Staurosporine (1/3/6 hour) treated Jurkat cells using Cleaved Caspase 3; p17 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO. RGAM002 ).

  • FC experiment of Jurkat using 68773-1-Ig

    FC experiment of Jurkat using 68773-1-Ig

    1x10^6 Jurkat cells were intracellularly stained with 0.2 μg Cleaved Caspase 3; p17 Monoclonal antibody (68773-1-Ig, Clone:2F7B8) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1), and 0.2 μg KLH (66360-1-Ig, Clone: T1F8D3F10). Cells were fixed with 4% PFA .

经过测试的应用

Positive WB detected inJurkat cells, A2780 cells, HepG2 cells, HSC-T6 cells, Ramos cells
Positive IF detected in1 μM Staurosporine (1/3/6 hour) treated Jurkat cells
Positive FC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68773-1-Ig targets Cleaved Caspase 3; p17 in WB, IF, FC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IF, FC, ELISA Application Description
Tested Reactivity Human, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name caspase 3, apoptosis-related cysteine peptidase
Synonyms Apopain, CASP 3, CASP3, Caspase 3, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight17 kDa
GenBank Accession NumberNM_032991
Gene Symbol CASP3
Gene ID (NCBI) 836
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42574
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17 of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3, it can recognize the 17 kDa cleaved-caspase 3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).

实验方案

Product Specific Protocols
WB protocol for Cleaved Caspase 3; p17 antibody 68773-1-IgDownload protocol
IF protocol for Cleaved Caspase 3; p17 antibody 68773-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols