• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 80138-2-RR

    WB analysis using 80138-2-RR

    Non-treated and GM-CSF treated TF-1 cells were subjected to SDS PAGE followed by western blot with 80138-2-RR (Phospho-STAT5A (Ser726)/STAT5B (Ser731) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • IF Staining of TF-1 using 80138-2-RR

    IF Staining of TF-1 using 80138-2-RR

    Immunofluorescent analysis of (4% PFA) fixed GM-CSF treated TF-1 cells using Phospho-STAT5A (Ser726)/STAT5B (Ser731) antibody (80138-2-RR, Clone: 240709D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of TF-1 using 80138-2-RR

    IF Staining of TF-1 using 80138-2-RR

    Immunofluorescent analysis of (4% PFA) fixed GM-CSF treated TF-1 cells using Phospho-STAT5A (Ser726)/STAT5B (Ser731) antibody (80138-2-RR, Clone: 240709D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of TF-1 using 80138-2-RR

    FC experiment of TF-1 using 80138-2-RR

    1x10^6 untreated TF-1 cells (dash lines) and 100 nM Calyculin A (30 minutes) treated TF-1 cells (full lines) were intracellularly stained with 0.4 μg Phospho-STAT5A (Ser726)/STAT5B (Ser731) Recombinant antibody (80138-2-RR, Clone: 240709D6, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR005). Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9, blue) was parallel stained as control. Cells were fixed with 4% PFA.

Phospho-STAT5A (Ser726)/STAT5B (Ser731) Recombinant antibody

Phospho-STAT5A (Ser726)/STAT5B (Ser731) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 80138-2-RR

产品说明书

CloneNo. 240709D6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

p STAT5A Ser726, p STAT5A (Ser726), p STAT5A, p STAT5 (Ser726), Phospho STAT5 (Ser726)

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inGM-CSF treated TF-1 cells
Positive IF/ICC detected inGM-CSF treated TF-1 cells
Positive FC (Intra) detected in100 nM Calyculin A (30 minutes) treated TF-1 cells
Planning an IHC experiment? We recommend our IHCeasy STAT5A Ready-To-Use IHC Kit. STAT5A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80138-2-RR targets Phospho-STAT5A (Ser726)/STAT5B (Ser731) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 signal transducer and activator of transcription 5A
别名 p STAT5A Ser726, p STAT5A (Ser726), p STAT5A, p STAT5 (Ser726), Phospho STAT5 (Ser726)
计算分子量 794 aa, 92 kDa
观测分子量90-95 kDa
GenBank蛋白编号BC027036
基因名称 STAT5A
Gene ID (NCBI) 6776
RRIDAB_3670468
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP42229
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT5 proteins consisting of STAT5A and STAT5B are the main targets of IL-3 signaling. Upon cytokine stimulation, JAK2 phosphorylates STAT5 and the phosphorylated STAT5 proteins dimerize and translocate into the nucleus, where, by binding DNA, they activate target genes including c-fos. In addition to this JAK-catalyzed tyrosine phosphorylation, STAT5 may undergo serine phosphorylation in the carboxy-terminal P(M)SP site in response to prolactin. (PMID: 15795318)

实验方案

Product Specific Protocols
WB protocol for Phospho-STAT5A (Ser726)/STAT5B (Ser731) antibody 80138-2-RRDownload protocol
IF protocol for Phospho-STAT5A (Ser726)/STAT5B (Ser731) antibody 80138-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols