验证数据展示
经过测试的应用
Positive FC (Intra) detected in | GM-CSF treated TF-1 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-80138-2 targets Phospho-STAT5A (Ser726)/STAT5B (Ser731) in FC (Intra) applications and shows reactivity with human samples.
经测试应用 | FC (Intra) Application Description |
经测试反应性 | human |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | signal transducer and activator of transcription 5A |
别名 | STAT5A, STAT5, MGF, 240709D6 |
计算分子量 | 794 aa, 92 kDa |
观测分子量 | 90-95 kDa |
GenBank蛋白编号 | BC027036 |
基因名称 | STAT5A |
Gene ID (NCBI) | 6776 |
偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
最大激发/发射波长 | 654 nm / 674 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P42229 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STAT5 proteins consisting of STAT5A and STAT5B are the main targets of IL-3 signaling. Upon cytokine stimulation, JAK2 phosphorylates STAT5 and the phosphorylated STAT5 proteins dimerize and translocate into the nucleus, where, by binding DNA, they activate target genes including c-fos. In addition to this JAK-catalyzed tyrosine phosphorylation, STAT5 may undergo serine phosphorylation in the carboxy-terminal P(M)SP site in response to prolactin. (PMID: 15795318)