验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | GM-CSF treated TF-1 cells |
Planning an IHC experiment? We recommend our IHCeasy STAT5A Ready-To-Use IHC Kit. STAT5A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80138-2-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-80138-2 targets Phospho-STAT5A (Ser726)/STAT5B (Ser731) in FC (Intra) applications and shows reactivity with human samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | signal transducer and activator of transcription 5A |
| Synonyms | STAT5A, STAT5, MGF, 240709D6 |
| Calculated Molecular Weight | 794 aa, 92 kDa |
| Observed Molecular Weight | 90-95 kDa |
| GenBank Accession Number | BC027036 |
| Gene Symbol | STAT5A |
| Gene ID (NCBI) | 6776 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P42229 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STAT5 proteins consisting of STAT5A and STAT5B are the main targets of IL-3 signaling. Upon cytokine stimulation, JAK2 phosphorylates STAT5 and the phosphorylated STAT5 proteins dimerize and translocate into the nucleus, where, by binding DNA, they activate target genes including c-fos. In addition to this JAK-catalyzed tyrosine phosphorylation, STAT5 may undergo serine phosphorylation in the carboxy-terminal P(M)SP site in response to prolactin. (PMID: 15795318)