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Phospho-STAT5A (Ser726) Recombinant antibody

Phospho-STAT5A (Ser726) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240709D6

Cat no : 80138-2-RR

Print datasheet

Synonyms

p STAT5A Ser726, p STAT5A (Ser726), p STAT5A, p STAT5 (Ser726), Phospho STAT5 (Ser726)

验证数据展示

  • WB analysis using 80138-2-RR

    WB analysis using 80138-2-RR

    Non-treated and GM-CSF treated TF-1 cells were subjected to SDS PAGE followed by western blot with 80138-2-RR (Phospho-STAT5A (Ser726) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • IF Staining of TF-1 using 80138-2-RR

    IF Staining of TF-1 using 80138-2-RR

    Immunofluorescent analysis of (4% PFA) fixed GM-CSF treated TF-1 cells using Phospho-STAT5A (Ser726) antibody (80138-2-RR, Clone: 240709D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of TF-1 using 80138-2-RR

    FC experiment of TF-1 using 80138-2-RR

    1x10^6 untreated TF-1 cells (dash lines) and 100 nM Calyculin A (30 minutes) treated TF-1 cells (full lines) were intracellularly stained with 0.4 μg Phospho-STAT5A (Ser726) Recombinant antibody (80138-2-RR, Clone: 240709D6, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR005). Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9, blue) was parallel stained as control. Cells were fixed with 4% PFA.

经过测试的应用

Positive WB detected inGM-CSF treated TF-1 cells
Positive IF/ICC detected inGM-CSF treated TF-1 cells
Positive FC (Intra) detected in100 nM Calyculin A (30 minutes) treated TF-1 cells
Planning an IHC experiment? We recommend our IHCeasy STAT5A Ready-To-Use IHC Kit. STAT5A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80138-2-RR targets Phospho-STAT5A (Ser726) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name signal transducer and activator of transcription 5A
Synonyms p STAT5A Ser726, p STAT5A (Ser726), p STAT5A, p STAT5 (Ser726), Phospho STAT5 (Ser726)
Calculated Molecular Weight 794 aa, 92 kDa
Observed Molecular Weight90-95 kDa
GenBank Accession NumberBC027036
Gene Symbol STAT5A
Gene ID (NCBI) 6776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP42229
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT5 proteins consisting of STAT5A and STAT5B are the main targets of IL-3 signaling. Upon cytokine stimulation, JAK2 phosphorylates STAT5 and the phosphorylated STAT5 proteins dimerize and translocate into the nucleus, where, by binding DNA, they activate target genes including c-fos. In addition to this JAK-catalyzed tyrosine phosphorylation, STAT5 may undergo serine phosphorylation in the carboxy-terminal P(M)SP site in response to prolactin. (PMID: 15795318)

实验方案

Product Specific Protocols
WB protocol for Phospho-STAT5A (Ser726) antibody 80138-2-RRDownload protocol
IF protocol for Phospho-STAT5A (Ser726) antibody 80138-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols