验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, ROS1728 cells, HepG2 cells, RAW264.7 cells |
| Positive IHC detected in | human liver cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue, MCF-7 cells, HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
80174-1-RR targets PARP1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | PARP1 fusion protein Ag4193 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | poly (ADP-ribose) polymerase 1 |
| 别名 | ARTD1, ADPRT1, ADPRT 1, ADPRT, ADP-ribosyltransferase diphtheria toxin-like 1 |
| 计算分子量 | 1014 aa, 113 kDa |
| 观测分子量 | 113-116, 89 kDa |
| GenBank蛋白编号 | BC037545 |
| 基因名称 | PARP1 |
| Gene ID (NCBI) | 142 |
| RRID | AB_2918872 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P09874 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PARP1 antibody 80174-1-RR | Download protocol |
| IHC protocol for PARP1 antibody 80174-1-RR | Download protocol |
| IF protocol for PARP1 antibody 80174-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Hazard Mater Exposure to 6PPD-Q induces dysfunctions of ovarian granulosa cells: Its potential role in PCOS | ||
Int J Biol Macromol Rosa rugosa polysaccharide induces autophagy-mediated apoptosis in human cervical cancer cells via the PI3K/AKT/mTOR pathway. | ||
Asian J Androl Safety of low-intensity extracorporeal shock wave therapy in prostate disorders: in vitro and in vivo evidence | ||
Cancer Biomark MKRN1/2 serve as tumor suppressors in renal clear cell carcinoma by regulating the expression of p53 | ||
PLoS One St-N, a novel alkaline derivative of stevioside, reverses docetaxel resistance by targeting lysosomes in vitro and in vivo |
