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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 80174-1-RR (same clone as 80174-1-PBS)

    WB analysis using 80174-1-RR (same clone as 80174-1-PBS)

    Jurkat cells treated with Staurosporin were subjected to SDS PAGE followed by western blot with 80174-1-RR (PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 80174-1-RR (same clone as 80174-1-PBS)

    IHC staining of human liver cancer using 80174-1-RR (same clone as 80174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80174-1-RR (PARP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PARP1 antibody included
  • IF Staining of mouse testis using 80174-1-RR (same clone as 80174-1-PBS)

    IF Staining of mouse testis using 80174-1-RR (same clone as 80174-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 80174-1-RR (same clone as 80174-1-PBS)

    IF Staining of mouse testis using 80174-1-RR (same clone as 80174-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 80174-1-RR (same clone as 80174-1-PBS)

    IHC staining of mouse testis using 80174-1-RR (same clone as 80174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80174-1-RR (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PARP1 antibody included
  • IF Staining of HepG2 using 80174-1-RR (same clone as 80174-1-PBS)

    IF Staining of HepG2 using 80174-1-RR (same clone as 80174-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80174-1-RR

    Affinity and Kinetic Characterization of 80174-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80174-1-RR against Human PARP1 were performed. The affinity constant is 0.639 nM.

PARP1 Recombinant antibody, PBS Only

PARP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 80174-1-PBS

产品说明书

CloneNo. 3N19

宿主/亚型

Rabbit / IgG

种属反应性

rat, mouse, human

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

ARTD1, ADPRT1, ADPRT 1, ADPRT, ADP-ribosyltransferase diphtheria toxin-like 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PARP1 Ready-To-Use IHC Kit. PARP1 primary antibody included.

产品信息

80174-1-PBS targets PARP1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 rat, mouse, human
免疫原 PARP1 fusion protein Ag4193 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 poly (ADP-ribose) polymerase 1
别名 ARTD1, ADPRT1, ADPRT 1, ADPRT, ADP-ribosyltransferase diphtheria toxin-like 1
计算分子量 1014 aa, 113 kDa
观测分子量113-116, 89 kDa
GenBank蛋白编号BC037545
基因名称 PARP1
Gene ID (NCBI) 142
RRIDAB_2918872
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09874
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.