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IHCeasy PARP1 Ready-To-Use IHC Kit

PARP1 Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat No : KHC1069

Synonyms

ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL

Validation Data Gallery

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC1069 (PARP1 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC1069 (PARP1 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using KHC1069 (PARP1 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using KHC1069 (PARP1 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC1069 (PARP1 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC1069 (PARP1 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using KHC1069 (PARP1 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using KHC1069 (PARP1 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using KHC1069 (PARP1 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using KHC1069 (PARP1 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using KHC1069 (PARP1 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using KHC1069 (PARP1 IHC Kit).

  • Components in IHCeasy ready-to-use IHC kit.

Product Information

KHC1069 is a ready-to-use IHC kit for staining of PARP1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy PARP1 Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL