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C1QBP Recombinant antibody

C1QBP Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3K20

Cat no : 80490-1-RR

Print datasheet

Synonyms

gC1Q R, GC1QBP, GC1q R protein, Complement component 1 Q subcomponent-binding protein, mitochondrial, ASF/SF2-associated protein p32

验证数据展示

  • WB analysis using 80490-1-RR

    WB analysis using 80490-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80490-1-RR (C1QBP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 80490-1-RR

    IHC staining of human tonsillitis using 80490-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80490-1-RR (C1QBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C1QBP antibody included
  • IHC staining of human tonsillitis using 80490-1-RR

    IHC staining of human tonsillitis using 80490-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80490-1-RR (C1QBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C1QBP antibody included
  • IF Staining of HeLa using 80490-1-RR

    IF Staining of HeLa using 80490-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using C1QBP antibody (80490-1-RR, Clone: 3K20 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

  • FC experiment of HeLa using 80490-1-RR

    FC experiment of HeLa using 80490-1-RR

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human C1QBP (80490-1-RR, Clone:3K20) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Rabbit IgG (KFA002), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 80490-1-RR

    Affinity and Kinetic Characterization of 80490-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80490-1-RR against Human C1QBP were performed. The affinity constant is 0.776 nM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy C1QBP Ready-To-Use IHC Kit. C1QBP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80490-1-RR targets C1QBP in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen C1QBP fusion protein Ag19773 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name complement component 1, q subcomponent binding protein
Synonyms gC1Q R, GC1QBP, GC1q R protein, Complement component 1 Q subcomponent-binding protein, mitochondrial, ASF/SF2-associated protein p32
Calculated Molecular Weight 282 aa, 31 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC013731
Gene Symbol C1QBP
Gene ID (NCBI) 708
RRIDAB_2918896
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07021
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1QBP, also named as gC1q receptor (gC1qR), p32, p33, and hyaluronan-binding protein 1 (HABP1), is a protein initially copurified with splicing factor SF2 (PMID: 1830244). The protein is synthesized as a pro-protein of 282 amino acids (aa) that is post-translationally processed by removal of the initial 73 aa to a mature protein of 209 aa (PMID: 8262387). C1QBP is an evolutionary conserved and ubiquitously expressed multifunctional protein and has been reported to be a predominantly mitochondrial matrix protein involved in inflammation and infection processes, mitochondrial ribosome biogenesis, regulation of apoptosis and nuclear transcription, and pre-mRNA splicing (PMID: 28942965).

实验方案

Product Specific Protocols
WB protocol for C1QBP antibody 80490-1-RRDownload protocol
IHC protocol for C1QBP antibody 80490-1-RRDownload protocol
IF protocol for C1QBP antibody 80490-1-RRDownload protocol
FC protocol for C1QBP antibody 80490-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols