• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CHMP4B Recombinant antibody

CHMP4B Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2N22

Cat No : 81691-2-RR

Print datasheet

Synonyms

C20orf178, CHMP4B, chromatin modifying protein 4B, CTPP3, dJ553F4.4, hSnf7 2, hVps32 2, Shax1, SNF7, SNF7 2, Vps32 2

验证数据展示

  • WB analysis using 81691-2-RR

    WB analysis using 81691-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81691-2-RR (CHMP4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity experiments of 81691-2-RR

    Affinity experiments of 81691-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81691-2-RR against Human CHMP4B were performed. The affinity constant is 36.7 nM.

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HuH-7 cells, C2C12 cells
Planning an IHC experiment? We recommend our IHCeasy CHMP4B Ready-To-Use IHC Kit. CHMP4B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 81691-2-RR is CHMP4B Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen CHMP4B fusion protein Ag4544 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromatin modifying protein 4B
Synonyms C20orf178, CHMP4B, chromatin modifying protein 4B, CTPP3, dJ553F4.4, hSnf7 2, hVps32 2, Shax1, SNF7, SNF7 2, Vps32 2
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC033859
Gene Symbol CHMP4B
Gene ID (NCBI) 128866
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9H444
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).

实验方案

Product Specific Protocols
WB protocol for CHMP4B antibody 81691-2-RRDownload protocol
FC protocol for CHMP4B antibody 81691-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols