• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

MRPS16 Recombinant antibody

MRPS16 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

11D12

Cat no : 82741-2-RR

Print datasheet

Synonyms

MRP S16, COXPD2, CGI-132, CGI132, CGI 132

验证数据展示

  • WB analysis of HeLa using 82741-2-RR

    WB analysis of HeLa using 82741-2-RR

    WB result of MRPS16 antibody (82741-2-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS16 transfected HeLa cells.

  • WB analysis using 82741-2-RR

    WB analysis using 82741-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82741-2-RR (MRPS16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82741-2-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 82741-2-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82741-2-RR (MRPS16 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82741-2-RR

    Affinity and Kinetic Characterization of 82741-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82741-2-RR against Human MRPS16 were performed. The affinity constant is 0.616 nM.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, HT-1080 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82741-2-RR targets MRPS16 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen MRPS16 fusion protein Ag9749 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitochondrial ribosomal protein S16
Synonyms MRP S16, COXPD2, CGI-132, CGI132, CGI 132
Calculated Molecular Weight 137 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC021106
Gene Symbol MRPS16
Gene ID (NCBI) 51021
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y3D3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS16 antibody 82741-2-RRDownload protocol
IHC protocol for MRPS16 antibody 82741-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols