• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

cGAS Recombinant antibody

cGAS Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H1

Cat no : 82869-1-RR

Print datasheet

Synonyms

h-cGAS, EC:2.7.7.86, cGAMP synthase, C6orf150, 2'3'-cGAMP synthase

验证数据展示

  • WB analysis using 82869-1-RR

    WB analysis using 82869-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82869-1-RR (cGAS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 82869-1-RR

    FC experiment of A431 using 82869-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug cGAS Recombinant antibody (82869-1-RR, Clone:1H1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of A431 using 82869-1-RR

    IF Staining of A431 using 82869-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using cGAS antibody (82869-1-RR, Clone: 1H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A431 using 82869-1-RR

    IF Staining of A431 using 82869-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using cGAS antibody (82869-1-RR, Clone: 1H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 82869-1-RR

    Affinity and Kinetic Characterization of 82869-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82869-1-RR against Human cGAS were performed. The affinity constant is 0.124 nM.

经过测试的应用

Positive WB detected inHeLa cells, HL-60 cells, THP-1 cells, MCF-7 cells, NK-92 cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82869-1-RR targets cGAS in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen cGAS fusion protein Ag23455 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromosome 6 open reading frame 150
Synonyms h-cGAS, EC:2.7.7.86, cGAMP synthase, C6orf150, 2'3'-cGAMP synthase
Calculated Molecular Weight 496 aa, 54 kDa
Observed Molecular Weight62 kDa
GenBank Accession NumberBC113608
Gene Symbol cGAS
Gene ID (NCBI) 115004
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N884
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.

实验方案

Product Specific Protocols
WB protocol for cGAS antibody 82869-1-RRDownload protocol
IF protocol for cGAS antibody 82869-1-RRDownload protocol
FC protocol for cGAS antibody 82869-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols