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TRIM62 Recombinant antibody

TRIM62 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230195G5

Cat no : 82892-1-RR

Print datasheet

Synonyms

Tripartite motif-containing protein 62, tripartite motif containing 62, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM62, 230195G5

验证数据展示

  • IF Staining of U2OS using 82892-1-RR

    IF Staining of U2OS using 82892-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TRIM62 antibody (82892-1-RR, Clone: 230195G5 ) at dilution of 1:150 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 82892-1-RR

    FC experiment of HeLa using 82892-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human TRIM62 (82892-1-RR, Clone:230195G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82892-1-RR

    Affinity and Kinetic Characterization of 82892-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82892-1-RR against Human TRIM62 were performed. The affinity constant is 90.8 pM.

经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82892-1-RR targets TRIM62 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen TRIM62 fusion protein Ag7143 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tripartite motif-containing 62
Synonyms Tripartite motif-containing protein 62, tripartite motif containing 62, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM62, 230195G5
Calculated Molecular Weight 54 kDa
GenBank Accession NumberBC001222
Gene Symbol TRIM62
Gene ID (NCBI) 55223
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BVG3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM62(Tripartite motif-containing protein 62) belongs to the TRIM/RBCC family. TRIM proteins are involved in a broad range of biological processes, including some that underlie genetic disorders, neurological disorders and cancers. TRIM62 is shown to promote the release of HIV from infected cells, and a TRIM62 protein that has a mutation in its RING domain was more active than the native protein in promoting HIV release(PMID:18836477).

实验方案

Product Specific Protocols
IF protocol for TRIM62 antibody 82892-1-RRDownload protocol
FC protocol for TRIM62 antibody 82892-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols