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  • Featured Product
  • KD/KO Validated

PAK7 Recombinant antibody

PAK7 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230201A10

Cat No : 82935-1-RR

Print datasheet

Synonyms

KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7

验证数据展示

  • WB analysis of HeLa using 82935-1-RR

    WB analysis of HeLa using 82935-1-RR

    WB result of PAK7 antibody (82935-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK7 transfected HeLa cells.

  • IF Staining of U-251 using 82935-1-RR

    IF Staining of U-251 using 82935-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PAK7 antibody (82935-1-RR, Clone: 230201A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis using 82935-1-RR

    WB analysis using 82935-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82935-1-RR (PAK7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, SH-SY5Y cells
Positive IF detected inU-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82935-1-RR targets PAK7 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen PAK7 fusion protein Ag29190 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name p21 protein (Cdc42/Rac)-activated kinase 7
Synonyms KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7
Calculated Molecular Weight 719 aa, 81 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC024179
Gene Symbol PAK7
Gene ID (NCBI) 57144
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P286
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAK7(p21-activated kinase 7) is also named as KIAA1264, PAK5 and belongs to the STE Ser/Thr protein kinase family. It plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival.

实验方案

Product Specific Protocols
WB protocol for PAK7 antibody 82935-1-RRDownload protocol
IF protocol for PAK7 antibody 82935-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols