验证数据展示
经过测试的应用
| Positive IF/ICC detected in | Neuro-2a cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82935 targets PAK7 in IF/ICC applications and shows reactivity with human samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | PAK7 fusion protein Ag29190 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | p21 protein (Cdc42/Rac)-activated kinase 7 |
| Synonyms | PAK4/6/7, PAK 5, p21-activated kinase 7, p21-activated kinase 5, KIAA1264 |
| Calculated Molecular Weight | 719 aa, 81 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC024179 |
| Gene Symbol | PAK7 |
| Gene ID (NCBI) | 57144 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9P286 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAK7, also known as PAK5, is a newly found but scarcely researched type II PAKs (PAK4, 5, 6). PAK7 was originally found in the brain, which promotes filopodia formation in nerve cells. Recently, studies have found that the expression level of PAK7 significantly increased in colorectal cancer, ovarian cancer, gastric cancer, neuroglioma, hepatocellular carcinoma, pancreatic cancer, osteosarcoma, and breast cancer. PAK7 plays an important role in tumorigenesis, invasion, and metastasis, mainly related to its mechanism of regulating cytoskeleton, inhibiting apoptosis, and promoting proliferation. The calculated molecular weight of PAK7 is 80 kDa (PMID: 29805709, PMID: 34165002).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PAK7 antibody CL488-82935 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |