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  • Featured Product
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PAK7 Recombinant antibody

PAK7 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230201A10

Cat no : 82935-1-RR

Print datasheet

Synonyms

PAK4/6/7, PAK 5, p21-activated kinase 7, p21-activated kinase 5, KIAA1264

验证数据展示

  • WB analysis of HeLa using 82935-1-RR

    WB analysis of HeLa using 82935-1-RR

    WB result of PAK7 antibody (82935-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK7 transfected HeLa cells.

  • IF Staining of U-251 using 82935-1-RR

    IF Staining of U-251 using 82935-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PAK7 antibody (82935-1-RR, Clone: 230201A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis using 82935-1-RR

    WB analysis using 82935-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82935-1-RR (PAK7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of K-562 using 82935-1-RR

    FC experiment of K-562 using 82935-1-RR

    (blue)1x10^6 K-562 cells were intracellularly stained with 0.25 ug Anti-Human PAK7 (82935-1-RR, Clone:230201A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, SH-SY5Y cells
Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inK-562 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82935-1-RR targets PAK7 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen PAK7 fusion protein Ag29190 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name p21 protein (Cdc42/Rac)-activated kinase 7
Synonyms PAK4/6/7, PAK 5, p21-activated kinase 7, p21-activated kinase 5, KIAA1264
Calculated Molecular Weight 719 aa, 81 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC024179
Gene Symbol PAK7
Gene ID (NCBI) 57144
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P286
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAK7, also known as PAK5, is a newly found but scarcely researched type II PAKs (PAK4, 5, 6). PAK7 was originally found in the brain, which promotes filopodia formation in nerve cells. Recently, studies have found that the expression level of PAK7 significantly increased in colorectal cancer, ovarian cancer, gastric cancer, neuroglioma, hepatocellular carcinoma, pancreatic cancer, osteosarcoma, and breast cancer. PAK7 plays an important role in tumorigenesis, invasion, and metastasis, mainly related to its mechanism of regulating cytoskeleton, inhibiting apoptosis, and promoting proliferation. The calculated molecular weight of PAK7 is 80 kDa (PMID: 29805709, PMID: 34165002).

实验方案

Product Specific Protocols
WB protocol for PAK7 antibody 82935-1-RRDownload protocol
IF protocol for PAK7 antibody 82935-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols