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  • Featured Product
  • KD/KO Validated

PAK7 Recombinant antibody, PBS Only

PAK7 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230201A10

Cat no : 82935-1-PBS

Print datasheet

Synonyms

PAK4/6/7, PAK 5, p21-activated kinase 7, p21-activated kinase 5, KIAA1264

验证数据展示

  • WB analysis using 82935-1-RR (same clone as 82935-1-PBS)

    WB analysis using 82935-1-RR (same clone as 82935-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82935-1-RR (PAK7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82935-1-RR (same clone as 82935-1-PBS)

    WB analysis of HeLa using 82935-1-RR (same clone as 82935-1-PBS)

    WB result of PAK7 antibody (82935-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK7 transfected HeLa cells. This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 82935-1-RR (same clone as 82935-1-PBS)

    IF Staining of U-251 using 82935-1-RR (same clone as 82935-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PAK7 antibody (82935-1-RR, Clone: 230201A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 82935-1-RR (same clone as 82935-1-PBS)

    FC experiment of K-562 using 82935-1-RR (same clone as 82935-1-PBS)

    (blue)1x10^6 K-562 cells were intracellularly stained with 0.25 ug Anti-Human PAK7 (82935-1-RR, Clone:230201A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

产品信息

82935-1-PBS targets PAK7 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen PAK7 fusion protein Ag29190 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name p21 protein (Cdc42/Rac)-activated kinase 7
Synonyms PAK4/6/7, PAK 5, p21-activated kinase 7, p21-activated kinase 5, KIAA1264
Calculated Molecular Weight 719 aa, 81 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC024179
Gene Symbol PAK7
Gene ID (NCBI) 57144
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P286
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAK7, also known as PAK5, is a newly found but scarcely researched type II PAKs (PAK4, 5, 6). PAK7 was originally found in the brain, which promotes filopodia formation in nerve cells. Recently, studies have found that the expression level of PAK7 significantly increased in colorectal cancer, ovarian cancer, gastric cancer, neuroglioma, hepatocellular carcinoma, pancreatic cancer, osteosarcoma, and breast cancer. PAK7 plays an important role in tumorigenesis, invasion, and metastasis, mainly related to its mechanism of regulating cytoskeleton, inhibiting apoptosis, and promoting proliferation. The calculated molecular weight of PAK7 is 80 kDa (PMID: 29805709, PMID: 34165002).