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验证数据展示

  • WB analysis using 82935-1-RR (same clone as 82935-1-PBS)

    WB analysis using 82935-1-RR (same clone as 82935-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82935-1-RR (PAK7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82935-1-RR (same clone as 82935-1-PBS)

    WB analysis of HeLa using 82935-1-RR (same clone as 82935-1-PBS)

    WB result of PAK7 antibody (82935-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK7 transfected HeLa cells. This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 82935-1-RR (same clone as 82935-1-PBS)

    FC experiment of K-562 using 82935-1-RR (same clone as 82935-1-PBS)

    (blue)1x10^6 K-562 cells were intracellularly stained with 0.25 ug Anti-Human PAK7 (82935-1-RR, Clone:230201A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PAK7 Recombinant antibody, PBS Only

PAK7 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82935-1-PBS

产品说明书

CloneNo. 230201A10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

PAK4/6/7, 230201A10, EC:2.7.11.1, KIAA1264, p21-activated kinase 5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82935-1-PBS targets PAK7 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 PAK7 fusion protein Ag29190 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 p21 protein (Cdc42/Rac)-activated kinase 7
别名 PAK4/6/7, 230201A10, EC:2.7.11.1, KIAA1264, p21-activated kinase 5
计算分子量 719 aa, 81 kDa
观测分子量70 kDa
GenBank蛋白编号BC024179
基因名称 PAK7
Gene ID (NCBI) 57144
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9P286
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAK7, also known as PAK5, is a newly found but scarcely researched type II PAKs (PAK4, 5, 6). PAK7 was originally found in the brain, which promotes filopodia formation in nerve cells. Recently, studies have found that the expression level of PAK7 significantly increased in colorectal cancer, ovarian cancer, gastric cancer, neuroglioma, hepatocellular carcinoma, pancreatic cancer, osteosarcoma, and breast cancer. PAK7 plays an important role in tumorigenesis, invasion, and metastasis, mainly related to its mechanism of regulating cytoskeleton, inhibiting apoptosis, and promoting proliferation. The calculated molecular weight of PAK7 is 80 kDa (PMID: 29805709, PMID: 34165002).