MTF2 Recombinant antibody
MTF2 Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
230138D10
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, Jurkat cells, HeLa cells, HepG2 cells |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82944-1-RR targets MTF2 in WB, IF, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | MTF2 fusion protein Ag9384 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | metal response element binding transcription factor 2 |
Synonyms | dJ976O13.2, hPCl2, M96, MTF2, PCL2, Polycomb like protein 2, RP5 976O13.1 |
Calculated Molecular Weight | 536 aa, 61 kDa |
Observed Molecular Weight | 55 kDa, 60 kDa, 75 kDa |
GenBank Accession Number | BC010013 |
Gene Symbol | MTF2 |
Gene ID (NCBI) | 22823 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9Y483 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTF2, also named as Metal-response element-binding transcription factor 2, is a 593 amino acid protein. It exists several isoforms and the range of the molecular weight of isoforms are about 55-60 kDa and 29 kDa. MTF2 contains 2 PHD-type zinc fingers and belongs to the Polycomblike family. MTF2 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity and regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation.
实验方案
Product Specific Protocols | |
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WB protocol for MTF2 antibody 82944-1-RR | Download protocol |
IF protocol for MTF2 antibody 82944-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |