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验证数据展示

  • WB analysis using 82944-1-RR (same clone as 82944-1-PBS)

    WB analysis using 82944-1-RR (same clone as 82944-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82944-1-RR (MTF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82944-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82944-1-RR (same clone as 82944-1-PBS)

    IF Staining of HeLa using 82944-1-RR (same clone as 82944-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MTF2 antibody (82944-1-RR, Clone: 230138D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82944-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 82944-1-RR (same clone as 82944-1-PBS)

    FC experiment of HEK-293 using 82944-1-RR (same clone as 82944-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug MTF2 Recombinant antibody (82944-1-RR, Clone:230138D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82944-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82944-1-RR

    Affinity and Kinetic Characterization of 82944-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82944-1-RR against Human MTF2 were performed. The affinity constant is 0.398 nM.

MTF2 Recombinant antibody, PBS Only

MTF2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82944-1-PBS

产品说明书

CloneNo. 230138D10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

MTF 2, Metal-response element-binding transcription factor 2, Metal-response element DNA-binding protein M96, M96, hPCl2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82944-1-PBS targets MTF2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 MTF2 fusion protein Ag9384 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 metal response element binding transcription factor 2
别名 MTF 2, Metal-response element-binding transcription factor 2, Metal-response element DNA-binding protein M96, M96, hPCl2
计算分子量 536 aa, 61 kDa
观测分子量55 kDa, 60 kDa, 75 kDa
GenBank蛋白编号BC010013
基因名称 MTF2
Gene ID (NCBI) 22823
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y483
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MTF2, also named as Metal-response element-binding transcription factor 2, is a 593 amino acid protein. It exists several isoforms and the range of the molecular weight of isoforms are about 55-60 kDa and 29 kDa. MTF2 contains 2 PHD-type zinc fingers and belongs to the Polycomblike family. MTF2 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity and regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation.