验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, Jurkat cells, HeLa cells, HepG2 cells |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HEK-293 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82944-1-RR targets MTF2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | human |
免疫原 | MTF2 fusion protein Ag9384 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | metal response element binding transcription factor 2 |
别名 | MTF 2, Metal-response element-binding transcription factor 2, Metal-response element DNA-binding protein M96, M96, hPCl2 |
计算分子量 | 536 aa, 61 kDa |
观测分子量 | 55 kDa, 60 kDa, 75 kDa |
GenBank蛋白编号 | BC010013 |
基因名称 | MTF2 |
Gene ID (NCBI) | 22823 |
RRID | AB_3670687 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q9Y483 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTF2, also named as Metal-response element-binding transcription factor 2, is a 593 amino acid protein. It exists several isoforms and the range of the molecular weight of isoforms are about 55-60 kDa and 29 kDa. MTF2 contains 2 PHD-type zinc fingers and belongs to the Polycomblike family. MTF2 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity and regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation.
实验方案
Product Specific Protocols | |
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WB protocol for MTF2 antibody 82944-1-RR | Download protocol |
IF protocol for MTF2 antibody 82944-1-RR | Download protocol |
FC protocol for MTF2 antibody 82944-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |