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IFT140 Recombinant antibody

IFT140 Recombinant Antibody for WB, ELISA, IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA, IF/ICC

Conjugate

Unconjugated

CloneNo.

230231D5

Cat No : 82947-7-RR

Print datasheet

Synonyms

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog, 230231D5

验证数据展示

  • WB analysis of HeLa using 82947-7-RR

    WB analysis of HeLa using 82947-7-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 82947-7-RR

    WB analysis of HepG2 using 82947-7-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 82947-7-RR

    IF Staining of hTERT-RPE1 using 82947-7-RR

    Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT140 antibody (82947-7-RR, Clone: 230231D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Affinity experiments of 82947-7-RR

    Affinity experiments of 82947-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82947-7 -RR against Human IFT140 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IF/ICC detected inStarvation treated hTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 82947-7-RR is IFT140 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA, IF/ICC Application Description
Tested Reactivity Human
Immunogen IFT140 fusion protein Ag11119 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name intraflagellar transport 140 homolog (Chlamydomonas)
Synonyms WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog, 230231D5
Calculated Molecular Weight 1462 aa, 165 kDa
Observed Molecular Weight150-165 kDa
GenBank Accession NumberBC035577
Gene Symbol IFT140
Gene ID (NCBI) 9742
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96RY7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localized to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports (PMID: 20368623, 22282595).

实验方案

Product Specific Protocols
WB protocol for IFT140 antibody 82947-7-RRDownload protocol
IF protocol for IFT140 antibody 82947-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols