CoraLite® Plus 488-conjugated IFT140 Recombinant antibody

IFT140 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-82947-7

产品说明书

CloneNo. 230231D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells
For other applications, we recommend the unconjugated version of this antibody, 82947-7-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82947-7 targets IFT140 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 IFT140 fusion protein Ag11119 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 intraflagellar transport 140 homolog (Chlamydomonas)
别名
计算分子量 1462 aa, 165 kDa
观测分子量150-165 kDa
GenBank蛋白编号BC035577
基因名称 IFT140
Gene ID (NCBI) 9742
RRIDAB_3673148
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96RY7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localized to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports (PMID: 20368623, 22282595).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IFT140 antibody CL488-82947-7Download protocol
Standard Protocols
Click here to view our Standard Protocols
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