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CoraLite® Plus 488-conjugated IFT140 Recombinant antibody

IFT140 Uni-rAbTM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230231D5

Cat no : CL488-82947-7

Print datasheet

Synonyms

验证数据展示

  • IF Staining of hTERT-RPE1 using CL488-82947-7

    IF Staining of hTERT-RPE1 using CL488-82947-7

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite® Plus 488 IFT140 antibody (CL488-82947-7, Clone: 230231D5 ) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).

经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells
For other applications, we recommend the unconjugated version of this antibody, 82947-7-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-82947-7 is IFT140 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen IFT140 fusion protein Ag11119 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name intraflagellar transport 140 homolog (Chlamydomonas)
Synonyms
Calculated Molecular Weight 1462 aa, 165 kDa
Observed Molecular Weight150-165 kDa
GenBank Accession NumberBC035577
Gene Symbol IFT140
Gene ID (NCBI) 9742
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96RY7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localized to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports (PMID: 20368623, 22282595).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IFT140 antibody CL488-82947-7Download protocol
Standard Protocols
Click here to view our Standard Protocols