验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells |
Positive IF/ICC detected in | Starvation treated hTERT-RPE1 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82947-7-RR targets IFT140 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | Human |
免疫原 | IFT140 fusion protein Ag11119 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | intraflagellar transport 140 homolog (Chlamydomonas) |
别名 | WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog, 230231D5 |
计算分子量 | 1462 aa, 165 kDa |
观测分子量 | 150-165 kDa |
GenBank蛋白编号 | BC035577 |
基因名称 | IFT140 |
Gene ID (NCBI) | 9742 |
RRID | AB_3670689 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | Q96RY7 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localized to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports (PMID: 20368623, 22282595).
实验方案
Product Specific Protocols | |
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WB protocol for IFT140 antibody 82947-7-RR | Download protocol |
IF protocol for IFT140 antibody 82947-7-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |