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IFT140 Recombinant antibody, PBS Only

IFT140 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230231D5

Cat no : 82947-7-PBS

Print datasheet

Synonyms

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog, 230231D5

验证数据展示

  • WB analysis of HeLa using 82947-7-RR (same clone as 82947-7-PBS)

    WB analysis of HeLa using 82947-7-RR (same clone as 82947-7-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82947-7-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 82947-7-RR (same clone as 82947-7-PBS)

    WB analysis of HepG2 using 82947-7-RR (same clone as 82947-7-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82947-7-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 82947-7-RR (same clone as 82947-7-PBS)

    IF Staining of hTERT-RPE1 using 82947-7-RR (same clone as 82947-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT140 antibody (82947-7-RR, Clone: 230231D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 82947-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82947-7-PBS

    Affinity and Kinetic Characterization of 82947-7-PBS

    Biolayer interferometry (BLl) kinetic assays of 82947-7 -RR against Human IFT140 were performed. The affinity constant is below 1 pM.

产品信息

The immunogen of 82947-7-PBS is IFT140 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen IFT140 fusion protein Ag11119 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name intraflagellar transport 140 homolog (Chlamydomonas)
Synonyms WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog, 230231D5
Calculated Molecular Weight 1462 aa, 165 kDa
GenBank Accession NumberBC035577
Gene Symbol IFT140
Gene ID (NCBI) 9742
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96RY7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C