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  • Featured Product
  • KD/KO Validated

BBS4 Recombinant antibody, PBS Only (Capture)

BBS4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230303C2

Cat no : 82961-1-PBS

Print datasheet

Synonyms

Bardet-Biedl syndrome 4 protein, Bardet Biedl syndrome 4, 230303C2

验证数据展示

  • WB analysis using 82961-1-RR (same clone as 82961-1-PBS)

    WB analysis using 82961-1-RR (same clone as 82961-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82961-1-RR (BBS4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82961-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82961-1-RR (same clone as 82961-1-PBS)

    FC experiment of HeLa using 82961-1-RR (same clone as 82961-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BBS4 Recombinant antibody (82961-1-RR, Clone:230303C2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82961-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 82961-1-RR (same clone as 82961-1-PBS)

    WB analysis of HEK-293 using 82961-1-RR (same clone as 82961-1-PBS)

    WB result of BBS4 antibody (82961-1-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-BBS4 transfected HEK-293 cells. This data was developed using the same antibody clone with 82961-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 82961-1-RR (same clone as 82961-1-PBS)

    IF Staining of hTERT-RPE1 using 82961-1-RR (same clone as 82961-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using BBS4 antibody (82961-1-RR, Clone: 230303C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 82961-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00130-1

    Cytometric bead array standard curve of MP00130-1

    Cytometric bead array standard curve ofMP00130-1, BBS4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82961-1-PBS. Detection antibody: 82961-3-PBS. Standard: Ag3430. Range: 0.313-20 ng/mL.

  • Affinity and Kinetic Characterization of 82961-1-RR

    Affinity and Kinetic Characterization of 82961-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82961-1-RR against Human BBS4 were performed. The affinity constant is below 1 pM.

82961-1-RR

产品信息

82961-1-PBS targets BBS4 as part of a matched antibody pair:

MP00130-1: 82961-1-PBS capture and 82961-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen BBS4 fusion protein Ag3430 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Bardet-Biedl syndrome 4
Synonyms Bardet-Biedl syndrome 4 protein, Bardet Biedl syndrome 4, 230303C2
Calculated Molecular Weight 519 aa, 58 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC027624
Gene Symbol BBS4
Gene ID (NCBI) 585
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96RK4
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).