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  • Featured Product
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BBS4 Recombinant antibody

BBS4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230303C2

Cat no : 82961-1-RR

Print datasheet

Synonyms

Bardet-Biedl syndrome 4 protein, Bardet Biedl syndrome 4, 230303C2

验证数据展示

  • WB analysis using 82961-1-RR

    WB analysis using 82961-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82961-1-RR (BBS4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 82961-1-RR

    FC experiment of HeLa using 82961-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BBS4 Recombinant antibody (82961-1-RR, Clone:230303C2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HEK-293 using 82961-1-RR

    WB analysis of HEK-293 using 82961-1-RR

    WB result of BBS4 antibody (82961-1-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-BBS4 transfected HEK-293 cells.

  • IF Staining of hTERT-RPE1 using 82961-1-RR

    IF Staining of hTERT-RPE1 using 82961-1-RR

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using BBS4 antibody (82961-1-RR, Clone: 230303C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

  • Affinity and Kinetic Characterization of 82961-1-RR

    Affinity and Kinetic Characterization of 82961-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82961-1-RR against Human BBS4 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, SH-SY5Y cells
Positive IF/ICC detected inhTERT-RPE1 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82961-1-RR targets BBS4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen BBS4 fusion protein Ag3430 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Bardet-Biedl syndrome 4
Synonyms Bardet-Biedl syndrome 4 protein, Bardet Biedl syndrome 4, 230303C2
Calculated Molecular Weight 519 aa, 58 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC027624
Gene Symbol BBS4
Gene ID (NCBI) 585
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96RK4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).

实验方案

Product Specific Protocols
WB protocol for BBS4 antibody 82961-1-RRDownload protocol
IF protocol for BBS4 antibody 82961-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols