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验证数据展示

  • IF Staining of hTERT-RPE1 using CL488-82961

    IF Staining of hTERT-RPE1 using CL488-82961

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite® Plus 488 BBS4 antibody (CL488-82961, Clone: 230303C2 ) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).

  • FC experiment of HeLa using CL488-82961

    FC experiment of HeLa using CL488-82961

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated BBS4 Recombinant antibody (CL488-82961, Clone:230303C2)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated BBS4 Recombinant antibody

BBS4 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-82961

产品说明书

CloneNo. 230303C2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

Bardet-Biedl syndrome 4 protein, Bardet Biedl syndrome 4, 230303C2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 82961-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82961 targets BBS4 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 BBS4 fusion protein Ag3430 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Bardet-Biedl syndrome 4
别名 Bardet-Biedl syndrome 4 protein, Bardet Biedl syndrome 4, 230303C2
计算分子量 519 aa, 58 kDa
观测分子量60 kDa
GenBank蛋白编号BC027624
基因名称 BBS4
Gene ID (NCBI) 585
RRIDAB_3673154
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96RK4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BBS4 antibody CL488-82961Download protocol
FC protocol for CL Plus 488 BBS4 antibody CL488-82961Download protocol
Standard Protocols
Click here to view our Standard Protocols