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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated BBS4 Recombinant antibody

BBS4 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230303C2

Cat no : CL488-82961

Print datasheet

Synonyms

Bardet-Biedl syndrome 4 protein, Bardet Biedl syndrome 4, 230303C2

验证数据展示

  • IF Staining of hTERT-RPE1 using CL488-82961

    IF Staining of hTERT-RPE1 using CL488-82961

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite® Plus 488 BBS4 antibody (CL488-82961, Clone: 230303C2 ) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).

  • FC experiment of HeLa using CL488-82961

    FC experiment of HeLa using CL488-82961

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated BBS4 Recombinant antibody (CL488-82961, Clone:230303C2)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 82961-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82961 targets BBS4 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen BBS4 fusion protein Ag3430 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Bardet-Biedl syndrome 4
Synonyms Bardet-Biedl syndrome 4 protein, Bardet Biedl syndrome 4, 230303C2
Calculated Molecular Weight 519 aa, 58 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC027624
Gene Symbol BBS4
Gene ID (NCBI) 585
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96RK4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BBS4 antibody CL488-82961Download protocol
FC protocol for CL Plus 488 BBS4 antibody CL488-82961Download protocol
Standard Protocols
Click here to view our Standard Protocols