N-cadherin Recombinant antibody
N-cadherin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
230259H5
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells |
Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat brain tissue |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82968-1-RR targets N-cadherin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
Tested Reactivity | human |
Immunogen | Recombinant protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | cadherin 2, type 1, N-cadherin (neuronal) |
Synonyms | CDHN, CDH2, CD325, Cadherin-2, Cadherin 2 |
Calculated Molecular Weight | 100 kDa |
Observed Molecular Weight | 130 kDa |
GenBank Accession Number | NM_001792.4 |
Gene Symbol | N-cadherin |
Gene ID (NCBI) | 1000 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P19022-1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for N-cadherin antibody 82968-1-RR | Download protocol |
IHC protocol for N-cadherin antibody 82968-1-RR | Download protocol |
IF protocol for N-cadherin antibody 82968-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |