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N-cadherin Recombinant antibody, PBS Only

N-cadherin Recombinant Antibody for WB, IF, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230259H5

Cat No : 82968-1-PBS

Print datasheet

Synonyms

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin

验证数据展示

  • WB analysis using 82968-1-RR (same clone as 82968-1-PBS)

    WB analysis using 82968-1-RR (same clone as 82968-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82968-1-RR (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82968-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82968-1-RR (same clone as 82968-1-PBS)

    FC experiment of HeLa using 82968-1-RR (same clone as 82968-1-PBS)

    1x10^6 HeLa cells were surface stained with 0.25 ug N-cadherin Recombinant antibody (82968-1-RR, Clone: 230259H5) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 82968-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82968-1-RR (same clone as 82968-1-PBS)

    IF Staining of HeLa using 82968-1-RR (same clone as 82968-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82968-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy N-cadherin Ready-To-Use IHC Kit. N-cadherin primary antibody included.

产品信息

82968-1-PBS targets N-cadherin in WB, IF, FC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF, FC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Synonyms Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin
Calculated Molecular Weight100 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberNM_001792.4
Gene Symbol CDH2
Gene ID (NCBI) 1000
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19022-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also includes E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).