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验证数据展示

  • WB analysis using 82968-1-RR

    WB analysis using 82968-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82968-1-RR (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82968-1-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 82968-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IF Staining of HeLa using 82968-1-RR

    IF Staining of HeLa using 82968-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of A549 using 82968-1-RR

    IF Staining of A549 using 82968-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 82968-1-RR

    Affinity and Kinetic Characterization of 82968-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82968-1-RR against Human N-cadherin were performed. The affinity constant is 0.464 nM.

N-cadherin Recombinant antibody

N-cadherin Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82968-1-RR

Print datasheet

CloneNo. 230259H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CDHN, CDH2, CD325, Cadherin-2, Cadherin 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy N-cadherin Ready-To-Use IHC Kit. N-cadherin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82968-1-RR targets N-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Synonyms CDHN, CDH2, CD325, Cadherin-2, Cadherin 2
Calculated Molecular Weight100 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberNM_001792.4
Gene Symbol N-cadherin
Gene ID (NCBI) 1000
RRIDAB_3670715
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19022-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also includes E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).

实验方案

Product Specific Protocols
WB protocol for N-cadherin antibody 82968-1-RRDownload protocol
IHC protocol for N-cadherin antibody 82968-1-RRDownload protocol
IF protocol for N-cadherin antibody 82968-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols