CoraLite® Plus 488-conjugated N-cadherin Recombinant antibody

N-cadherin Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-82968

产品说明书

CloneNo. 230259H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

CDHN, CDH2, CD325, Cadherin-2, Cadherin 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHeLa cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy N-cadherin Ready-To-Use IHC Kit. N-cadherin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82968-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82968 targets N-cadherin in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 2, type 1, N-cadherin (neuronal)
别名 CDHN, CDH2, CD325, Cadherin-2, Cadherin 2
计算分子量100 kDa
观测分子量130 kDa
GenBank蛋白编号NM_001792.4
基因名称 N-cadherin
Gene ID (NCBI) 1000
RRIDAB_3673157
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP19022-1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also includes E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 N-cadherin antibody CL488-82968Download protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...