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LIMK2 Recombinant antibody

LIMK2 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230283D10

Cat No : 82977-1-RR

Print datasheet

Synonyms

LIMK-2, LIMK 2, LIM domain kinase 2, EC:2.7.11.1, 230283D10

验证数据展示

  • IF Staining of HepG2 using 82977-1-RR

    IF Staining of HepG2 using 82977-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LIMK2 antibody (82977-1-RR, Clone: 230283D10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity experiments of 82977-1-RR

    Affinity experiments of 82977-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82977-1-RR against Human LIMK2 were performed. The affinity constant is 4.03 nM.

经过测试的应用

Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82977-1-RR targets LIMK2 in IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen LIMK2 fusion protein Ag3011 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name LIM domain kinase 2
Synonyms LIMK-2, LIMK 2, LIM domain kinase 2, EC:2.7.11.1, 230283D10
Calculated Molecular Weight 686 aa, 78 kDa
GenBank Accession NumberBC013051
Gene Symbol LIMK2
Gene ID (NCBI) 3985
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP53671
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for LIMK2 antibody 82977-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols