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MTAP Recombinant antibody

MTAP Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240030E5

Cat no : 83005-5-RR

Print datasheet

Synonyms

MTA phosphorylase, MSAP, EC:2.4.2.28, c86fus, 5'-methylthioadenosine phosphorylase

验证数据展示

  • IF Staining of HeLa using 83005-5-RR

    IF Staining of HeLa using 83005-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTAP antibody (83005-5-RR, Clone: 240030E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • FC experiment of HeLa using 83005-5-RR

    FC experiment of HeLa using 83005-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MTAP Recombinant antibody (83005-5-RR, Clone:240030E5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83005-5-RR

    Affinity and Kinetic Characterization of 83005-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83005-5-RR against Human MTAP were performed. The affinity constant is 1.54 nM.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83005-5-RR is MTAP Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen MTAP fusion protein Ag2051 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name methylthioadenosine phosphorylase
Synonyms MTA phosphorylase, MSAP, EC:2.4.2.28, c86fus, 5'-methylthioadenosine phosphorylase
Calculated Molecular Weight 283 aa, 31 kDa
GenBank Accession NumberBC018625
Gene Symbol MTAP
Gene ID (NCBI) 4507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13126
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.

实验方案

Product Specific Protocols
IF protocol for MTAP antibody 83005-5-RRDownload protocol
FC protocol for MTAP antibody 83005-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols