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验证数据展示

  • IF Staining of HeLa using 83005-5-RR (same clone as 83005-5-PBS)

    IF Staining of HeLa using 83005-5-RR (same clone as 83005-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTAP antibody (83005-5-RR, Clone: 240030E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83005-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83005-5-RR (same clone as 83005-5-PBS)

    FC experiment of HeLa using 83005-5-RR (same clone as 83005-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MTAP Recombinant antibody (83005-5-RR, Clone:240030E5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83005-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83005-5-RR

    Affinity and Kinetic Characterization of 83005-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83005-5-RR against Human MTAP were performed. The affinity constant is 1.54 nM.

MTAP Recombinant antibody, PBS Only

MTAP Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA
Cat No. 83005-5-PBS

产品说明书

CloneNo. 240030E5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, Indirect ELISA

别名

MTA phosphorylase, MSAP, EC:2.4.2.28, c86fus, 5'-methylthioadenosine phosphorylase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83005-5-PBS targets MTAP in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 MTAP fusion protein Ag2051 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methylthioadenosine phosphorylase
别名 MTA phosphorylase, MSAP, EC:2.4.2.28, c86fus, 5'-methylthioadenosine phosphorylase
计算分子量 283 aa, 31 kDa
GenBank蛋白编号BC018625
基因名称 MTAP
Gene ID (NCBI) 4507
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13126
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.