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MTAP Recombinant antibody, PBS Only

MTAP Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240030E5

Cat no : 83005-5-PBS

Print datasheet

Synonyms

MTA phosphorylase, MSAP, EC:2.4.2.28, c86fus, 5'-methylthioadenosine phosphorylase

验证数据展示

  • IF Staining of HeLa using 83005-5-RR (same clone as 83005-5-PBS)

    IF Staining of HeLa using 83005-5-RR (same clone as 83005-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTAP antibody (83005-5-RR, Clone: 240030E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83005-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83005-5-RR (same clone as 83005-5-PBS)

    FC experiment of HeLa using 83005-5-RR (same clone as 83005-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MTAP Recombinant antibody (83005-5-RR, Clone:240030E5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83005-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83005-5-RR

    Affinity and Kinetic Characterization of 83005-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83005-5-RR against Human MTAP were performed. The affinity constant is 1.54 nM.

产品信息

The immunogen of 83005-5-PBS is MTAP Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen MTAP fusion protein Ag2051 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name methylthioadenosine phosphorylase
Synonyms MTA phosphorylase, MSAP, EC:2.4.2.28, c86fus, 5'-methylthioadenosine phosphorylase
Calculated Molecular Weight 283 aa, 31 kDa
GenBank Accession NumberBC018625
Gene Symbol MTAP
Gene ID (NCBI) 4507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13126
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.