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验证数据展示

  • IF Staining of HeLa using 83005-5-RR

    IF Staining of HeLa using 83005-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTAP antibody (83005-5-RR, Clone: 240030E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • FC experiment of HeLa using 83005-5-RR

    FC experiment of HeLa using 83005-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MTAP Recombinant antibody (83005-5-RR, Clone:240030E5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83005-5-RR

    Affinity and Kinetic Characterization of 83005-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83005-5-RR against Human MTAP were performed. The affinity constant is 1.54 nM.

MTAP Recombinant antibody

MTAP Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 83005-5-RR

产品说明书

CloneNo. 240030E5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

MTA phosphorylase, MSAP, EC:2.4.2.28, c86fus, 5'-methylthioadenosine phosphorylase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83005-5-RR targets MTAP in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MTAP fusion protein Ag2051 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methylthioadenosine phosphorylase
别名 MTA phosphorylase, MSAP, EC:2.4.2.28, c86fus, 5'-methylthioadenosine phosphorylase
计算分子量 283 aa, 31 kDa
GenBank蛋白编号BC018625
基因名称 MTAP
Gene ID (NCBI) 4507
RRIDAB_3670751
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13126
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.

实验方案

Product Specific Protocols
IF protocol for MTAP antibody 83005-5-RRDownload protocol
FC protocol for MTAP antibody 83005-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols