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CD68 Recombinant antibody

CD68 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

230504G10

Cat no : 83014-5-RR

Print datasheet

Synonyms

Scard1, gp110, CD68 antigen, 230504G10

验证数据展示

  • WB analysis using 83014-5-RR

    WB analysis using 83014-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83014-5-RR (CD68 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 83014-5-RR

    IHC staining of mouse spleen using 83014-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD68 antibody included
  • IHC staining of mouse spleen using 83014-5-RR

    IHC staining of mouse spleen using 83014-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD68 antibody included
  • IF Staining of mouse spleen using 83014-5-RR

    IF Staining of mouse spleen using 83014-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 83014-5-RR

    IF Staining of mouse spleen using 83014-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83014-5-RR

    Affinity and Kinetic Characterization of 83014-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83014-5-RR against Mouse CD68 were performed. The affinity constant is 8.77 nM.

经过测试的应用

Positive WB detected inRAW 264.7 cells, J774A.1 cells, NR8383 cells
Positive IHC detected inmouse spleen tissue, mouse liver tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83014-5-RR targets CD68 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD68 antigen
Synonyms Scard1, gp110, CD68 antigen, 230504G10
Calculated Molecular Weight35 kDa
Observed Molecular Weight100-120 kDa
GenBank Accession NumberNM_001291058.1
Gene Symbol Cd68
Gene ID (NCBI) 12514
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP31996-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
WB protocol for CD68 antibody 83014-5-RRDownload protocol
IHC protocol for CD68 antibody 83014-5-RRDownload protocol
IF protocol for CD68 antibody 83014-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols