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Mouse CD68 Recombinant antibody, PBS Only

CD68 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230504G10

Cat no : 83014-5-PBS

Print datasheet

Synonyms

Scard1, gp110, CD68 antigen, 230504G10

验证数据展示

  • WB analysis using 83014-5-RR (same clone as 83014-5-PBS)

    WB analysis using 83014-5-RR (same clone as 83014-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83014-5-RR (CD68 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    IHC staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD68 antibody included
  • IHC staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    IHC staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD68 antibody included
  • IF Staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    IF Staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    IF Staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83014-5-RR

    Affinity and Kinetic Characterization of 83014-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83014-5-RR against Mouse CD68 were performed. The affinity constant is 8.77 nM.

Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

产品信息

The immunogen of 83014-5-PBS is CD68 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity mouse, rat
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD68 antigen
Synonyms Scard1, gp110, CD68 antigen, 230504G10
Calculated Molecular Weight35 kDa
Observed Molecular Weight100-120 kDa
GenBank Accession NumberNM_001291058.1
Gene Symbol Cd68
Gene ID (NCBI) 12514
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP31996-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.