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CD68 Recombinant antibody

CD68 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230504G10

Cat No : 83014-5-RR

Print datasheet

Synonyms

Cd68, CD68 antigen, gp110, Scard1

验证数据展示

  • WB analysis using 83014-5-RR

    WB analysis using 83014-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83014-5-RR (CD68 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRAW 264.7 cells, J774A.1 cells, NR8383 cells
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83014-5-RR is CD68 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity Mouse, Rat
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD68 antigen
Synonyms Cd68, CD68 antigen, gp110, Scard1
Calculated Molecular Weight35 kDa
Observed Molecular Weight100-120 kDa
GenBank Accession NumberNM_001291058.1
Gene Symbol Cd68
Gene ID (NCBI) 12514
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP31996-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
WB protocol for CD68 antibody 83014-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols