CD68 Recombinant antibody

CD68 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83014-5-RR

Print datasheet

CloneNo. 230504G10

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Scard1, gp110, CD68 antigen, 230504G10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inRAW 264.7 cells, J774A.1 cells, NR8383 cells
Positive IHC detected inmouse spleen tissue, mouse liver tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue, rat small intestine tissue
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83014-5-RR targets CD68 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen CD68 fusion protein Eg0787 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD68 antigen
Synonyms Scard1, gp110, CD68 antigen, 230504G10
Calculated Molecular Weight35 kDa
Observed Molecular Weight100-120 kDa
GenBank Accession NumberNM_001291058.1
Gene Symbol Cd68
Gene ID (NCBI) 12514
RRIDAB_3670757
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP31996-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
WB protocol for CD68 antibody 83014-5-RRDownload protocol
IHC protocol for CD68 antibody 83014-5-RRDownload protocol
IF protocol for CD68 antibody 83014-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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