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PTPN18 Recombinant antibody

PTPN18 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230154G8

Cat no : 83024-1-RR

Print datasheet

Synonyms

Tyrosine-protein phosphatase non-receptor type 18, PTP HSCF, EC:3.1.3.48, Brain-derived phosphatase, BDP1

验证数据展示

  • WB analysis using 83024-1-RR

    WB analysis using 83024-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83024-1-RR (PTPN18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 83024-1-RR

    FC experiment of HEK-293 using 83024-1-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug PTPN18 Recombinant antibody (83024-1-RR, Clone:230154G8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83024-1-RR

    Affinity and Kinetic Characterization of 83024-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83024-1-RR against Human PTPN18 were performed. The affinity constant is 7.48 nM.

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, MCF-7 cells, T-47D cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83024-1-RR targets PTPN18 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen PTPN18 fusion protein Ag34048 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 18 (brain-derived)
Synonyms Tyrosine-protein phosphatase non-receptor type 18, PTP HSCF, EC:3.1.3.48, Brain-derived phosphatase, BDP1
Calculated Molecular Weight 460 aa, 50 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC052800
Gene Symbol PTPN18
Gene ID (NCBI) 26469
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99952
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN18 (also called PTP-HSCF or BDP1), a member of the protein tyrosine phosphatase superfamily, has been reported to be a tumor suppressor in breast cancer by dephosphorylating HER2 and suppressing cell proliferation and invasion. In addition to the well-established role of PTPN18 in breast cancer, our previous study found that PTPN18 and CSK cooperate to inhibit the events triggered by SRC kinases. (PMID: 35982039)

实验方案

Product Specific Protocols
WB protocol for PTPN18 antibody 83024-1-RRDownload protocol
FC protocol for PTPN18 antibody 83024-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols