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CoraLite® Plus 750-conjugated PTPN18 Recombinant antibody

PTPN18 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230154G8

Cat no : CL750-83024

Print datasheet

Synonyms

Tyrosine-protein phosphatase non-receptor type 18, PTP HSCF, EC:3.1.3.48, Brain-derived phosphatase, BDP1

验证数据展示

  • WB analysis using CL750-83024

    WB analysis using CL750-83024

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-83024 (PTPN18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using CL750-83024

    FC experiment of MCF-7 using CL750-83024

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug CoraLite® Plus 750-conjugated PTPN18 Recombinant antibody (CL750-83024, Clone:230154G8)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells, T-47D cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 83024-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-83024 targets PTPN18 in WB, FC (Intra) applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human
Immunogen PTPN18 fusion protein Ag34048 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 18 (brain-derived)
Synonyms Tyrosine-protein phosphatase non-receptor type 18, PTP HSCF, EC:3.1.3.48, Brain-derived phosphatase, BDP1
Calculated Molecular Weight 460 aa, 50 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC052800
Gene Symbol PTPN18
Gene ID (NCBI) 26469
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99952
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN18 (also called PTP-HSCF or BDP1), a member of the protein tyrosine phosphatase superfamily, has been reported to be a tumor suppressor in breast cancer by dephosphorylating HER2 and suppressing cell proliferation and invasion. In addition to the well-established role of PTPN18 in breast cancer, our previous study found that PTPN18 and CSK cooperate to inhibit the events triggered by SRC kinases. (PMID: 35982039)

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 PTPN18 antibody CL750-83024Download protocol
Standard Protocols
Click here to view our Standard Protocols