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MTA3 Recombinant antibody, PBS Only (Capture)

MTA3 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230350D12

Cat No : 83042-3-PBS

Print datasheet

Synonyms

KIAA1266, MTA3

验证数据展示

  • WB analysis using 83042-3-RR (same clone as 83042-3-PBS)

    WB analysis using 83042-3-RR (same clone as 83042-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83042-3-RR (MTA3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83042-3-RR (same clone as 83042-3-PBS)

    FC experiment of U-251 using 83042-3-RR (same clone as 83042-3-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug MTA3 Recombinant antibody (83042-3-RR, Clone:230350D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 83042-3-RR (same clone as 83042-3-PBS)

    IF Staining of U-251 using 83042-3-RR (same clone as 83042-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (83042-3-RR, Clone: 230350D12 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00017-2

    Cytometric bead array standard curve of MP00017-2

    Cytometric bead array standard curve ofMP00017-2, MTA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83042-3-PBS. Detection antibody: 83042-1-PBS. Standard: Ag6400. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00017-3

    Cytometric bead array standard curve of MP00017-3

    Cytometric bead array standard curve ofMP00017-3, MTA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83042-3-PBS. Detection antibody: 83042-5-PBS. Standard: Ag6400. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83042-3-PBS

    Affinity and Kinetic Characterization of 83042-3-PBS

    Biolayer interferometry (BLl) kinetic assays of 83042-3-RR against Human MTA3 were performed. The affinity constant is below 1 pM.

产品信息

83042-3-PBS targets MTA3 as part of a matched antibody pair:

MP00017-2: 83042-3-PBS capture and 83042-1-PBS detection (validated in Cytometric bead array)

MP00017-3: 83042-3-PBS capture and 83042-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen MTA3 fusion protein Ag6400 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name metastasis associated 1 family, member 3
Synonyms KIAA1266, MTA3
Calculated Molecular Weight 68 kDa
GenBank Accession NumberBC053631
Gene Symbol MTA3
Gene ID (NCBI) 57504
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BTC8
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C