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验证数据展示

  • WB analysis using 83042-3-RR (same clone as 83042-3-PBS)

    WB analysis using 83042-3-RR (same clone as 83042-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83042-3-RR (MTA3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83042-3-RR (same clone as 83042-3-PBS)

    FC experiment of U-251 using 83042-3-RR (same clone as 83042-3-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug MTA3 Recombinant antibody (83042-3-RR, Clone:230350D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 83042-3-RR (same clone as 83042-3-PBS)

    IF Staining of U-251 using 83042-3-RR (same clone as 83042-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (83042-3-RR, Clone: 230350D12 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 83042-3-RR (same clone as 83042-3-PBS)

    WB analysis of MCF-7 using 83042-3-RR (same clone as 83042-3-PBS)

    WB result of MTA3 antibody (83042-3-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTA3 transfected MCF-7 cells. This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00017-2

    Sandwich ELISA standard curve of MP00017-2

    Sandwich ELISA standard curve of MP00017-2, Human MTA3 Recombinant Matched Antibody Pair - PBS only. 83042-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6400. 83042-1-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

  • Sample test of MP00017-2

    Sample test of MP00017-2

    Hela cell lysate was measured. The human MTA3 concentration of detected samples was determined to be 50.22 ng/mL (based on a 3.2 mg/mL extract load).

  • Cytometric bead array standard curve of MP00017-2

    Cytometric bead array standard curve of MP00017-2

    Cytometric bead array standard curve of MP00017-2, MTA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83042-3-PBS. Detection antibody: 83042-1-PBS. Standard: Ag6400. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00017-3

    Cytometric bead array standard curve of MP00017-3

    Cytometric bead array standard curve of MP00017-3, MTA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83042-3-PBS. Detection antibody: 83042-5-PBS. Standard: Ag6400. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83042-3-RR

    Affinity and Kinetic Characterization of 83042-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83042-3-RR against Human MTA3 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

MTA3 Recombinant antibody, PBS Only (Capture)

MTA3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83042-3-PBS

产品说明书

CloneNo. 230350D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

mta1, MTA 3, Metastasis-associated protein MTA3, 230350D12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83042-3-PBS targets MTA3 as part of a matched antibody pair:

MP00017-2: 83042-3-PBS capture and 83042-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00017-3: 83042-3-PBS capture and 83042-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 MTA3 fusion protein Ag6400 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 metastasis associated 1 family, member 3
别名 mta1, MTA 3, Metastasis-associated protein MTA3, 230350D12
计算分子量 68 kDa
观测分子量62 kDa, 68 kDa
GenBank蛋白编号BC053631
基因名称 MTA3
Gene ID (NCBI) 57504
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9BTC8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.