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MTA3 Recombinant antibody

MTA3 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230350D12

Cat No : 83042-3-RR

Print datasheet

Synonyms

KIAA1266, MTA3

验证数据展示

  • WB analysis using 83042-3-RR

    WB analysis using 83042-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83042-3-RR (MTA3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • FC experiment of U-251 using 83042-3-RR

    FC experiment of U-251 using 83042-3-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug MTA3 Recombinant antibody (83042-3-RR, Clone:230350D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of U-251 using 83042-3-RR

    IF Staining of U-251 using 83042-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (83042-3-RR, Clone: 230350D12 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83042-3-RR

    Affinity and Kinetic Characterization of 83042-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83042-3-RR against Human MTA3 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inJurkat cells, A375 cells, NIH/3T3 cells, C2C12 cells, MCF-7 cells, Raji cells
Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inU-251 cells
Positive FC detected inU-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83042-3-RR targets MTA3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen MTA3 fusion protein Ag6400 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name metastasis associated 1 family, member 3
Synonyms KIAA1266, MTA3
Calculated Molecular Weight 68 kDa
Observed Molecular Weight62 kDa, 68 kDa
GenBank Accession NumberBC053631
Gene Symbol MTA3
Gene ID (NCBI) 57504
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BTC8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.

实验方案

Product Specific Protocols
WB protocol for MTA3 antibody 83042-3-RRDownload protocol
IF protocol for MTA3 antibody 83042-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols