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CoraLite® Plus 750-conjugated MTA3 Recombinant antibody

MTA3 Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230350D12

Cat No : CL750-83042-3

Print datasheet

Synonyms

mta1, MTA 3, Metastasis-associated protein MTA3, 230350D12

验证数据展示

  • WB analysis using CL750-83042-3

    WB analysis using CL750-83042-3

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-83042-3 (MTA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of U-251 using CL750-83042-3

    FC experiment of U-251 using CL750-83042-3

    1x10^6 U-251 cells were intracellularly stained with 0.8 ug Coralite® Plus 750 Mta3 Recombinant Antibody (CL750-83042-3, Clone:230350D12)(red), or 0.8 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inMCF-7 cells, Raji cells
Positive FC (Intra) detected inU-251 cells
For other applications, we recommend the unconjugated version of this antibody, 83042-3-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-83042-3 targets MTA3 in WB, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen MTA3 fusion protein Ag6400 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name metastasis associated 1 family, member 3
Synonyms mta1, MTA 3, Metastasis-associated protein MTA3, 230350D12
Calculated Molecular Weight 68 kDa
Observed Molecular Weight62 kDa, 68 kDa
GenBank Accession NumberBC053631
Gene Symbol MTA3
Gene ID (NCBI) 57504
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BTC8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 MTA3 antibody CL750-83042-3Download protocol
Standard Protocols
Click here to view our Standard Protocols