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验证数据展示

  • WB analysis using 83042-3-RR (same clone as 83042-3-PBS)

    WB analysis using 83042-3-RR (same clone as 83042-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83042-3-RR (MTA3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83042-3-RR (same clone as 83042-3-PBS)

    FC experiment of U-251 using 83042-3-RR (same clone as 83042-3-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug MTA3 Recombinant antibody (83042-3-RR, Clone:230350D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 83042-3-RR (same clone as 83042-3-PBS)

    IF Staining of U-251 using 83042-3-RR (same clone as 83042-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (83042-3-RR, Clone: 230350D12 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 83042-3-RR (same clone as 83042-3-PBS)

    WB analysis of MCF-7 using 83042-3-RR (same clone as 83042-3-PBS)

    WB result of MTA3 antibody (83042-3-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTA3 transfected MCF-7 cells. This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00017-2

    Sandwich ELISA standard curve of MP00017-2

    Sandwich ELISA standard curve of MP00017-2, Human MTA3 Recombinant Matched Antibody Pair - PBS only. 83042-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6400. 83042-1-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

  • Sample test of MP00017-2

    Sample test of MP00017-2

    Hela cell lysate was measured. The human MTA3 concentration of detected samples was determined to be 50.22 ng/mL (based on a 3.2 mg/mL extract load).

  • Cytometric bead array standard curve of MP00017-2

    Cytometric bead array standard curve of MP00017-2

    Cytometric bead array standard curve of MP00017-2, MTA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83042-3-PBS. Detection antibody: 83042-1-PBS. Standard: Ag6400. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00017-3

    Cytometric bead array standard curve of MP00017-3

    Cytometric bead array standard curve of MP00017-3, MTA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83042-3-PBS. Detection antibody: 83042-5-PBS. Standard: Ag6400. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83042-3-RR

    Affinity and Kinetic Characterization of 83042-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83042-3-RR against Human MTA3 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

MTA3 Recombinant antibody, PBS Only (Capture)

MTA3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83042-3-PBS

Print datasheet

CloneNo. 230350D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

mta1, MTA 3, Metastasis-associated protein MTA3, 230350D12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

83042-3-PBS targets MTA3 as part of a matched antibody pair:

MP00017-2: 83042-3-PBS capture and 83042-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00017-3: 83042-3-PBS capture and 83042-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse
Immunogen MTA3 fusion protein Ag6400 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name metastasis associated 1 family, member 3
Synonyms mta1, MTA 3, Metastasis-associated protein MTA3, 230350D12
Calculated Molecular Weight 68 kDa
Observed Molecular Weight62 kDa, 68 kDa
GenBank Accession NumberBC053631
Gene Symbol MTA3
Gene ID (NCBI) 57504
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BTC8
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.