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Cytokeratin 13 Recombinant antibody

Cytokeratin 13 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230366E8

Cat no : 83058-4-RR

Print datasheet

Synonyms

KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13

验证数据展示

  • IF Staining of A431 using 83058-4-RR

    IF Staining of A431 using 83058-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Cytokeratin 13 antibody (83058-4-RR, Clone: 230366E8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A431 using 83058-4-RR

    FC experiment of A431 using 83058-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human KRT13 (83058-4-RR, Clone:230366E8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83058-4-RR

    Affinity and Kinetic Characterization of 83058-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83058-4-RR against Human Cytokeratin 13 were performed. The affinity constant is 1.65 nM.

经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT13 Ready-To-Use IHC Kit. KRT13 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83058-4-RR targets Cytokeratin 13 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity Human
Immunogen Cytokeratin 13 fusion protein Ag0217 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name keratin 13
Synonyms KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13
Calculated Molecular Weight 50 kDa
GenBank Accession NumberBC002661
Gene Symbol Cytokeratin 13
Gene ID (NCBI) 3860
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13646
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.

实验方案

Product Specific Protocols
IF protocol for Cytokeratin 13 antibody 83058-4-RRDownload protocol
FC protocol for Cytokeratin 13 antibody 83058-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols