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DDX39A Recombinant antibody

DDX39A Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230375F9

Cat no : 83083-5-RR

Print datasheet

Synonyms

DDX39, DDXL, BAT1L, BAT1, ATP-dependent RNA helicase DDX39A

验证数据展示

  • WB analysis using 83083-5-RR

    WB analysis using 83083-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83083-5-RR (DDX39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 83083-5-RR

    IHC staining of mouse kidney using 83083-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83083-5-RR (DDX39A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX39A antibody included
  • IF Staining of HepG2 using 83083-5-RR

    IF Staining of HepG2 using 83083-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX39 antibody (83083-5-RR, Clone: 230375F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • WB analysis using 83083-5-RR

    WB analysis using 83083-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83083-5-RR (DDX39A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 83083-5-RR

    WB analysis of HEK-293 using 83083-5-RR

    WB result of DDX39A antibody (83083-5-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX39A transfected HEK-293 cells.

  • FC experiment of Jurkat using 83083-5-RR

    FC experiment of Jurkat using 83083-5-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Anti-Human DDX39A (83083-5-RR, Clone:230375F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83083-5-RR

    Affinity and Kinetic Characterization of 83083-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83083-5-RR against Human DDX39A were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, mouse kidney tissue, HeLa cells, HepG2 cells, Raji cells, A431 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy DDX39A Ready-To-Use IHC Kit. DDX39A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83083-5-RR targets DDX39A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen DDX39A fusion protein Ag2311 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 39
Synonyms DDX39, DDXL, BAT1L, BAT1, ATP-dependent RNA helicase DDX39A
Calculated Molecular Weight 427 aa, 49 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC032128
Gene Symbol DDX39
Gene ID (NCBI) 10212
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00148
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX39A, also named the BAT1 protein, contains the nine conserved motifs that characterize the DEAD-box family of RNA-binding proteins. The family includes proteins found in all eukaryotic cell types, with considerable divergence in the sequences lying between the conserved motifs. Some of the motifs were known before the definition of the family and are responsible for binding to mRNA or ATP, or possess ATPase activity. Phylogenetic analyses have grouped BAT1 with the defining member of the DEAD-box family, eIF-4. This is a translation initiation factor required for the dissociation of stem/loop structures in mRNA at the ribosomes.

实验方案

Product Specific Protocols
WB protocol for DDX39A antibody 83083-5-RRDownload protocol
IHC protocol for DDX39A antibody 83083-5-RRDownload protocol
IF protocol for DDX39A antibody 83083-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols