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CoraLite® Plus 488-conjugated DDX39A Recombinant antibody

DDX39A Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83083-5

Print datasheet

CloneNo. 230375F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

DDX39, DDXL, BAT1L, BAT1, ATP-dependent RNA helicase DDX39A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -


经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy DDX39A Ready-To-Use IHC Kit. DDX39A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83083-5-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83083-5 targets DDX39A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen DDX39A fusion protein Ag2311 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 39
Synonyms DDX39, DDXL, BAT1L, BAT1, ATP-dependent RNA helicase DDX39A
Calculated Molecular Weight 427 aa, 49 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC032128
Gene Symbol DDX39
Gene ID (NCBI) 10212
RRIDAB_3673182
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00148
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX39A, also named the BAT1 protein, contains the nine conserved motifs that characterize the DEAD-box family of RNA-binding proteins. The family includes proteins found in all eukaryotic cell types, with considerable divergence in the sequences lying between the conserved motifs. Some of the motifs were known before the definition of the family and are responsible for binding to mRNA or ATP, or possess ATPase activity. Phylogenetic analyses have grouped BAT1 with the defining member of the DEAD-box family, eIF-4. This is a translation initiation factor required for the dissociation of stem/loop structures in mRNA at the ribosomes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DDX39A antibody CL488-83083-5Download protocol
Standard Protocols
Click here to view our Standard Protocols
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