IHCeasy DDX39A Ready-To-Use IHC Kit
DDX39A Ready-to-use reagent kit for IHC.
Reactivity
Human, Mouse
Sample Type
FFPE tissue
Cat No : KHC0928
Synonyms
BAT1, BAT1L, DDX39, DDX39A, DDXL, DEAD box protein 39, Nuclear RNA helicase URH49, URH49
Validation Data Gallery
Product Information
KHC0928 is a ready-to-use IHC kit for staining of DDX39A. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy DDX39A Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
DDX39A, also named the BAT1 protein, contains the nine conserved motifs that characterize the DEAD-box family of RNA binding proteins. The family includes proteins found in all eukaryotic cell types, with considerable divergence in the sequences lying between the conserved motifs.Some of the motifs were known prior to the definition of the family and are responsible for binding to mRNAor ATP, or possess ATPase activity. Phylogenetic analyses have grouped BAT1 with the defining member of the DEAD-box family, eIF-4. This is a translation initiation factor required for the dissociation of stem/loop structures in mRNA at the ribosomes. Primary antibody in this kit may cross-react with DDX39B.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | BAT1, BAT1L, DDX39, DDX39A, DDXL, DEAD box protein 39, Nuclear RNA helicase URH49, URH49 |
