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PPFIA3 Recombinant antibody, PBS Only (Capture)

PPFIA3 Uni-rAbTM Recombinant Antibody for WB, IP, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230459F6

Cat no : 83142-4-PBS

Print datasheet

Synonyms

PTPRF-interacting protein alpha-3, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3, Liprin-alpha-3, KIAA0654, 230459F6

验证数据展示

  • WB analysis using 83142-4-RR (same clone as 83142-4-PBS)

    WB analysis using 83142-4-RR (same clone as 83142-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83142-4-RR (PPFIA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83142-4-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 83142-4-RR (same clone as 83142-4-PBS)

    FC experiment of HEK-293 using 83142-4-RR (same clone as 83142-4-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug PPFIA3 Recombinant antibody (83142-4-RR, Clone:230459F6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83142-4-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83142-4-RR (same clone as 83142-4-PBS)

    FC experiment of U-251 using 83142-4-RR (same clone as 83142-4-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug PPFIA3 Recombinant antibody (83142-4-RR, Clone:230459F6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83142-4-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 83142-4-RR (same clone as 83142-4-PBS)

    IP experiment of mouse brain using 83142-4-RR (same clone as 83142-4-PBS)

    IP result of anti-PPFIA3 (IP:83142-4-RR, 4ug; Detection:83142-4-RR 1:4000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 83142-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00170-2

    Cytometric bead array standard curve of MP00170-2

    Cytometric bead array standard curve ofMP00170-2, PPFIA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83142-4-PBS. Detection antibody: 83142-3-PBS. Standard: Ag1971. Range: 1.25-20 ng/mL.

  • Cytometric bead array standard curve of MP00170-3

    Cytometric bead array standard curve of MP00170-3

    Cytometric bead array standard curve ofMP00170-3, PPFIA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83142-4-PBS. Detection antibody: 83142-2-PBS. Standard: Ag1971. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 83142-4-RR

    Affinity and Kinetic Characterization of 83142-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83142-4-RR against Human PPFIA3 were performed. The affinity constant is 0.131 nM.

Planning an IHC experiment? We recommend our IHCeasy PPFIA3 Ready-To-Use IHC Kit. PPFIA3 primary antibody included.

产品信息

83142-4-PBS targets PPFIA3 as part of a matched antibody pair:

MP00170-2: 83142-4-PBS capture and 83142-3-PBS detection (validated in Cytometric bead array)

MP00170-3: 83142-4-PBS capture and 83142-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IP, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PPFIA3 fusion protein Ag1971 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3
Synonyms PTPRF-interacting protein alpha-3, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3, Liprin-alpha-3, KIAA0654, 230459F6
Calculated Molecular Weight 1194 aa, 133 kDa
GenBank Accession NumberBC021255
Gene Symbol PPFIA3
Gene ID (NCBI) 8541
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75145
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C