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验证数据展示

  • Cytometric bead array standard curve of MP00188-1

    Cytometric bead array standard curve of MP00188-1

    Cytometric bead array standard curve of MP00188-1, USP8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83172-2-PBS. Detection antibody: 83172-1-PBS. Standard: Ag27143. Range: 1.25-20 ng/mL.

  • Sandwich ELISA standard curve of MP00188-1

    Sandwich ELISA standard curve of MP00188-1

    Sandwich ELISA standard curve of MP00188-1, Human USP8 Recombinant Matched Antibody Pair - PBS only. 83172-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag27143. 83172-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

  • Sample test of MP00188-1

    Sample test of MP00188-1

    The mean USP8 concentration was determined to be 41.15 ng/mL in MCF-7 cell extract based on a 4.30 mg/mL extract load and 47.12 ng/mL in K-562 cell extract based on a 4.50 mg/mL extract load.

  • WB analysis using 83172-2-RR (same clone as 83172-2-PBS)

    WB analysis using 83172-2-RR (same clone as 83172-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83172-2-RR (USP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83172-2-RR (same clone as 83172-2-PBS)

    IF Staining of U2OS using 83172-2-RR (same clone as 83172-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83172-2-RR (same clone as 83172-2-PBS)

    IF Staining of U2OS using 83172-2-RR (same clone as 83172-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83172-2-RR (same clone as 83172-2-PBS)

    IF Staining of HeLa using 83172-2-RR (same clone as 83172-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83172-2-RR (same clone as 83172-2-PBS)

    FC experiment of A549 using 83172-2-RR (same clone as 83172-2-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug USP8 Recombinant antibody (83172-2-RR, Clone:240016D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00188-2

    Cytometric bead array standard curve of MP00188-2

    Cytometric bead array standard curve of MP00188-2, USP8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83172-2-PBS. Detection antibody: 83172-3-PBS. Standard: Ag27143. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83172-2-RR

    Affinity and Kinetic Characterization of 83172-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83172-2-RR against Human USP8 were performed. The affinity constant is below 1 pM.

USP8 Recombinant antibody, PBS Only (Capture)

USP8 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83172-2-PBS

产品说明书

CloneNo. 240016D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Ubiquitin carboxyl-terminal hydrolase 8, KIAA0055, HumORF8, hUBPy, EC:3.4.19.12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy USP8 Ready-To-Use IHC Kit. USP8 primary antibody included.

产品信息

83172-2-PBS targets USP8 as part of a matched antibody pair:

MP00188-1: 83172-2-PBS capture and 83172-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00188-2: 83172-2-PBS capture and 83172-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 USP8 fusion protein Ag27143 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin specific peptidase 8
别名 Ubiquitin carboxyl-terminal hydrolase 8, KIAA0055, HumORF8, hUBPy, EC:3.4.19.12
计算分子量 1118 aa, 128 kDa
GenBank蛋白编号BC110590
基因名称 USP8
Gene ID (NCBI) 9101
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP40818
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C