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USP8 Recombinant antibody, PBS Only (Capture)

USP8 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240016D3

Cat No : 83172-2-PBS

Print datasheet

Synonyms

Ubiquitin carboxyl-terminal hydrolase 8, KIAA0055, HumORF8, hUBPy, EC:3.4.19.12

验证数据展示

  • WB analysis using 83172-2-RR (same clone as 83172-2-PBS)

    WB analysis using 83172-2-RR (same clone as 83172-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83172-2-RR (USP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83172-2-RR (same clone as 83172-2-PBS)

    IF Staining of U2OS using 83172-2-RR (same clone as 83172-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83172-2-RR (same clone as 83172-2-PBS)

    IF Staining of U2OS using 83172-2-RR (same clone as 83172-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using USP8 antibody (83172-2-RR, Clone: 240016D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83172-2-RR (same clone as 83172-2-PBS)

    FC experiment of A549 using 83172-2-RR (same clone as 83172-2-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug USP8 Recombinant antibody (83172-2-RR, Clone:240016D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83172-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00188-1

    Cytometric bead array standard curve of MP00188-1

    Cytometric bead array standard curve ofMP00188-1, USP8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83172-2-PBS. Detection antibody: 83172-1-PBS. Standard: Ag27143. Range: 1.25-20 ng/mL.

  • Cytometric bead array standard curve of MP00188-2

    Cytometric bead array standard curve of MP00188-2

    Cytometric bead array standard curve ofMP00188-2, USP8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83172-2-PBS. Detection antibody: 83172-3-PBS. Standard: Ag27143. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83172-2-RR

    Affinity and Kinetic Characterization of 83172-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83172-2-RR against Human USP8 were performed. The affinity constant is below 1 pM.

产品信息

83172-2-PBS targets USP8 as part of a matched antibody pair:

MP00188-1: 83172-2-PBS capture and 83172-1-PBS detection (validated in Cytometric bead array)

MP00188-2: 83172-2-PBS capture and 83172-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen USP8 fusion protein Ag27143 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin specific peptidase 8
Synonyms Ubiquitin carboxyl-terminal hydrolase 8, KIAA0055, HumORF8, hUBPy, EC:3.4.19.12
Calculated Molecular Weight 1118 aa, 128 kDa
GenBank Accession NumberBC110590
Gene Symbol USP8
Gene ID (NCBI) 9101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP40818
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C