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Rat IL-6 Recombinant antibody, PBS Only (Detector)

IL-6 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240003G3

Cat no : 83179-3-PBS

Print datasheet

Synonyms

IL6, interleukin 6, ILg6, IL 6, Ifnb2

验证数据展示

  • Sandwich ELISA standard curve of MP00264-2

    Sandwich ELISA standard curve of MP00264-2

    Sandwich ELISA standard curve of MP00264-2, Rat IL-6 Monoclonal Matched Antibody Pair - PBS only. 83179-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0800. 83179-3-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

  • Sample test of MP00264-2

    Sample test of MP00264-2

    Three rats were given an intraperitoneal injection of 100 μg/kg of LPS. All ratswere killed 4 hours after receiving LPS. Spleens were harvested and broken into individual cells by using homogenizer. Ratspleen conditioned media wascollected after culturing in 60 mL of RPMIsupplemented with 10% fetal bovine serum and stimulated with 1 μg/mL of LPS for 18 hours. An aliquot of the cell culture supernatant wasremoved, assayed for rat IL-6 and measured 6,449.6 pg/mL.

  • Sample test of MP00264-2

    Sample test of MP00264-2

    One rat spleen was cultured for 18 hours in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were stimulated with 10 μg/mL of PHA and 10 ng/mL of PMA. An aliquot of the cell culture supernatant was removed, assayed for rat IL-6 and measured 179.7 pg/mL.

  • Sample test of MP00264-2

    Sample test of MP00264-2

    Sixteen rat serum samples were evaluated for rat IL-6 in this assay. Fourteen samples were measured belowthe minimum standard of 62.5 pg/mL. The measured results of the two samples were 117.15 pg/mL and 133.51 pg/mL, respectively.

  • Cytometric bead array standard curve of MP00264-1

    Cytometric bead array standard curve of MP00264-1

    Cytometric bead array standard curve ofMP00264-1, Rat IL-6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83179-1-PBS. Detection antibody: 83179-3-PBS. Standard: Eg0800. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00264-2

    Cytometric bead array standard curve of MP00264-2

    Cytometric bead array standard curve ofMP00264-2, Rat IL-6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83179-2-PBS. Detection antibody: 83179-3-PBS. Standard: Eg0800. Range: 0.625-20 ng/mL.

产品信息

83179-3-PBS targets IL-6 as part of a matched antibody pair:

MP00264-1: 83179-1-PBS capture and 83179-3-PBS detection (validated in Cytometric bead array)

MP00264-2: 83179-2-PBS capture and 83179-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 6
Synonyms IL6, interleukin 6, ILg6, IL 6, Ifnb2
Calculated Molecular Weight24 kDa
GenBank Accession NumberNM_012589.2
Gene Symbol Il6
Gene ID (NCBI) 24498
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20607
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C